Long Oligo Synthesis

Long Oligos Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long-oligo synthesis. The techniques we have developed to purify long oligos are unmatched by other suppliers.

Long oligo synthesis. Failure sequences build up with increasing oligo length, and long oligos contain impurities that are, by definition, similar in properties to the desired sequence. If failure sequences are allowed to accumulate it is impossible to obtain pure oligos because the capping step in oligonucleotide synthesis cannot be 100% efficient. Long Oligo Synthesis. Gene Universal provides 81 to 150 base long oligo synthesis service. Desalted synthesized long oligos are shipped as dry powder. Synthesis scales up to 1 µmol are shipped in 3-4 business days. Product Synthesis Scale Length Price 25 nmole L-DNA oligo. Oligo Business Division. Macrogen offers an oligo synthesis service on various scales using high-quality raw materials and an optimized process. The synthesized oligos are used in various biology and medical fields such as DNA sequencing, PCR, SNP study, gene synthesis, NGS service, qPCR service, biochip and siRNA expression. Product Synthesis Scale Length Price 25 nmole L-DNA oligo: 25 nmole: 61-100 bases: $ 0.60 / base: 50 nmole L-DNA oligo: 50 nmole: 61-100 bases

Long Oligo Synthesis. General Biosystems provides 81 to 150 base long oligo synthesis service. Service Options. Product. Synthesis Scale. Length. Price. 25 nmole M-DNA oligo. 25 nmole. 81-150 bases. $ 0.60 / base. 50 nmole M-DNA oligo. 50 nmole. 91-150 bases. $ 0.70/ base. 250 nmole M-DNA oligo. 250 nmole. 101-150 bases. EXTREmer Oligos - Extremely long oligos up to 200 bases suitable for use in several molecular - and synthetic biology applications w10.0.27 | c9.0.90.06 PROD | u7.5.14 Reduced overall yield is expected as oligo length increases. Purified 99-mer with 5'-Fluorescein. Dharmacon RNA synthesis capabilities support the routine synthesis of long, chemically modified RNA oligonucleotides. UPLC and LC-MS analysis demonstrate the standard level of purity obtained. Synbio Technologies can provide high quality long RNA with modification or labeling, as well as customized lncRNA. Based on extensive experience in the oligo synthesis and the powerful Syno ® Synthesis Platform, we can chemically synthesize long RNA up to 100 bases. In addition, we can also biologically synthesize longer RNA like lncRNA.

Long Oligo Synthesis. GeneUniversal provides 81 to 125 base long oligo synthesis service. READ MORE. 96- & 384-Well Plate Synthesis. GeneUniversal provides high-throughput oligo synthesis in 96- and 384-well plate formats. READ MORE. 302-235-9792. sales@geneuniversal.com. Gene Synthesis. As oligonucleotide synthesis proceeds in the 3′→5′ direction, it becomes the 3′-most nucleotide of the oligo. Note: Initial oligo subunits are not limited to nucleotides A, C, T, and G. IDT can also fix modifications to the solid support when they are needed at the 3′ end of an oligo. BioServe India is an ISO 9001:2015 & DSIR certified lab and state-of-the-art research organization delivering high-quality products to match researchers’ needs. We offer a complete line of custom oligonucleotide synthesis and purification services for your research. BioServe oligos are synthesized on completely automated and high throughput MerMade IV and MerMade 192E Oligonucleotide. Oligo-Affinity Supports. Empty Synthesis Columns & Frits. Glen Unysupport. Universal Supports. Oligo-Affinity Supports.. Long Oligonucleotide Synthesis. 8 Product Results Sort by: Show per page. dA-CPG 2000 (20-2002).

When comparing oligo synthesis services, the O.D. should be compared (the final yield) and not the initial synthesis scale (in "nmol") which is the quantity of the starting material required before synthesis of the oligo. From this, the final "nmol" yield will vary on the length, sequence and molecular weight of the individual oligo. Flexible Synthesis Scales: 50 nmol, 100 nmol, 200 nmol, 1 µmol, 5 µmol, and 10 µmol. Bulk orders are available upon request Bulk orders are available upon request Experienced Team: scientists and staff with over 20 years of experience in oligo synthesis and modifications Bioneer offers a long oligo synthesis service for cloning, mutagenesis and other demanding applications. Bioneer synthesizes long oligos from 130 up to 200 bases (Exendamers) and provides them with Bio-RP or PAGE purification. Ordering long RNA oligos. The RNA Oligo Entry Tool makes ordering long RNA oligos easy. There is even a “Convert to RNA” feature that quickly switches an entire oligo from DNA to RNA. Upon placing an order, you will receive your Ultramer RNA Oligos in 6–8 business days, delivered dry, in tubes.

Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure ().The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical. All of the DNA synthesis reagents have an impact on good quality DNA synthesis, as are all the steps in the synthesis cycle – coupling, capping, oxidation and detritylation. However, for the successful synthesis of long oligos, some factors are absolutely critical, as noted below. GENEWIZ provides a suite of oligo synthesis options to meet the ever-changing needs of the molecular bench scientist. From oligos for Sanger sequencing and PCR to oligos modified with your choice of over 200 chemical modifications, our oligo synthesis services provide the high quality results you expect from GENEWIZ with the breadth of oligo synthesis expertise you need for your experiments. Our B-pure Oligonucleotide Synthesis protocol produces custom synthesis of affordable, high-quality long oligos up to 300 bases. For oligonucleotides longer than 80 bases the B-pure protocol is the standard synthesis method.

Long, high-quality oligos for demanding applications such as cloning, ddRNAi, homology-directed repair, and gene construction. Ultramer DNA Oligonucleotides are generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases. They are available single- or double-stranded and can be delivered in tubes or plates.