Mirna Synthesis

Home-> Products & Services-> Synthesis Platform-> miRNA Agomir & Antagomir: Quick Inquiry : miRNA Agomir & Antagomir. An antagomir (anti-miR, blockmir) is a small, single-strand, synthetic RNA with chemical modifications including 5’ end-(phosphorothioate)2, 3’ end-cholesterol, 3’ end-(phosphorothioate)4 and whole sequence 2’-methoxy group.

Mirna synthesis. MicroRNA (miRNA) mimics are innovative biomolecules useful for research in gene targeting, antisense and cell reprogramming approches miRNA mimics are innovative molecules designed for gene silencing approaches. miRNA mimics contain nonnatural or artificial double stranded miRNA-like RNA fragments. These RNA fragments are constructed to contain a sequence motif on its 5’-end that is. Purification of total RNA including miRNAs for comparison of miRNA expression levels with those of housekeeping reference genes or any other mRNA of interest can be challenging. Our miRNA isolation kits enable miRNA extraction from a variety of tissues and cells, including difficult-to-lyse tissues. Custom miRNA synthesis. Custom microRNA modulation tools. Modify an existing microRNA mimic or inhibitor, target a novel microRNA, or request an Accell microRNA Inhibitor or Stabilized Mimic for special applications.. Modulation of miRNA levels in both gain- and loss-of function experiments is a fundamental way to understand not only. A microRNA (abbreviated miRNA) is a small non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals and some viruses, that functions in RNA silencing and post-transcriptional regulation of gene expression. miRNAs function via base-pairing with complementary sequences within mRNA molecules. As a result, these mRNA molecules are silenced, by one or more of the following.

Creative Biogene is committed to providing outstanding miRNA products and comprehensive services to facilitate and simplify miRNA investigation. Creative Biogene is a global leading agomir/ antagomir service provider for all passionate scientific researchers and our comprehensive synthesis service is supported by Cutting-Edge Chemical Synthesis. mirna synthesis synthesis pathways and miRNAs for microRNA research. Search. Main menu. Skip to primary content.. on 10 g of whey protein ingestion. mTOR is a 289 kDa serine/threonine kinase downstream of Akt and stimulates protein synthesis through downstream activation of p70S6K and 4E-BP1, providing a key point of convergence for both. The Canonical Pathway of miRNA Biogenesis. The canonical biogenesis pathway is the dominant pathway by which miRNAs are processed. In this pathway, pri-miRNAs are transcribed from their genes and then processed into pre-miRNAs by the microprocessor complex, consisting of an RNA binding protein DiGeorge Syndrome Critical Region 8 (DGCR8) and a ribonuclease III enzyme, Drosha (). How It Works. The mRNAExpress mRNA Synthesis Kit workflow. The mRNAExpress mRNA Synthesis Kit has a straightforward workflow: Clone the cDNA of your gene-of-interest in the pMRNA XP mRNAExpress Vector (included in the Kit, but also available separately, # MR000PA-1); Tail-PCR amplify the in vitro transcription template from the pMRNA XP Vector with your insert cloned in.

MiRNA genes are transcribed in the nucleus, primarily by RNA polymerase II, as a primary miRNA (pri-miRNA), which is a long transcript (from a few hundred to tens of thousands of nucleotides) (Fig. 1). The primary miRNA is then converted into a miRNA precursor (pre-miRNA) by the Drosha-DGCR8 microprocessor complex (canonical pathway) . Mir-X miRNA qRT-PCR SYBR Kits are complete, dual-function systems for performing first-strand cDNA synthesis and quantitative PCR (qPCR) to precisely measure the level of your favorite miRNAs. The kits are available in economical, large-sized formats that provide 200 or 600 qPCR reactions, and each kit includes a Mir-X miRNA First-Strand. Recent scientific advances have revealed the synthesis pathways and the regulatory mechanisms of miRNAs in animals and plants. miRNA-based regulation is implicated in disease etiology and has been studied for treatment. Furthermore, several preclinical and clinical trials have been initiated for miRNA-based therapeutics. The miRCURY LNA RT Kit enables fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step. Using one universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared with systems that require miRNA-specific first-strand synthesis, adding convenience and saving you time.

MicroRNA Agomir/Antagomir Synthesis . AcceGen has pioneered the development of microRNA research and diagnostics tools with leading-edge services. We’re committed to paving new ways to meet all your needs for miRNA agomir/antagomir synthesis. Agomir is chemically-modified double-strand miRNA mimics. Its antisense strand is modified: 2 phosphorothioates at the 5′ end, 4 phosphorothioates at. MISSION® miRNA Product Offerings; Synonym: Custom miRNA, Human miRNA cDNA Library, Lenti TuDs, Lentiviral miRNA mimics, MISSION® 3′UTR Lenti GoClone, MISSION® Lentiviral miRNA inhibitors, MISSION® LightSwitch Luciferase Assay Reagent, MISSION® Target ID Library, MISSION® miRNA Human inhibitors, MISSION® miRNA Human mimics, MISSION® miRNA Workflow, Synthetic TuDs, Synthetic miRNA. Long RNA Oligonucleotide as Synthetic Pre-miRNA-Based shRNA . Bio-Synthesis provides synthesis of oligo ultramer and modification with flexible synthesis scales for your individual research needs. The ability to synthesize RNA oligos is critical to many laboratory techniques. The animal miRNA synthesis pathway. The microRNA (miRNA) genes are transcribed by RNA polymerase II (Pol II), which results in the production of a pri-miRNA. Drosha, along with DiGeorge syndrome critical region gene-8 (DGCR-8; Pasha in flies), mediates the initial processing step (primary processing) that produces a ~ 65 nucleotide (nt) pre-miRNA.

Bio-Synthesis supplies Custom RNA Oligo Synthesis services for a wide range of labels, modifications, scales and purities. We provide RNA synthesis for long RNA oligos up to 300 bases. RNA oligos are synthesized using the latest nucleic acid technology and are purified using RNase free PAGE electrophoresis combined with HPLC. Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution is designed to provide a streamlined workflow that allows for exceptional detection of multiple miRNA targets from a single sample. miRNA mimics & inhibitors. We provide chemically synthesized RNA oligonucleotides optimized to mimic or inhibit miRNA molecules. Custom synthesized miRNA mimics and inhibitors are available with different labels and can be linked to cholesterol to increase cellular uptake. The TaqMan Advanced miRNA cDNA Synthesis Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription. Universal RT primers recognize the universal sequences present on both the 5' and 3' extended ends of the mature miRNAs.

Table 2 TaqMan® Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007): 50 rxns Component Part No. Storage 10X Poly(A) Buffer 100032529 –20°C ATP, 10 mM 100032528 Poly(A) Enzyme, 5 U/µL 100029178 5X DNA Ligase Buffer 100029179 RNA Ligase, 10 U/µL 100029180 50% PEG 8000 100029181 25X Ligation Adaptor 100029182 10X RT Enzyme Mix 100029183